Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody | Boster M00532-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody

catalog :

M00532-1

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

DID-11

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

product information

SKU :

M00532-1

Product Name :

Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

DID-11

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IP, IF, IHC, ICC, WB

Application Details :

WB 1:1000-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .IP 1:30

Description :

Boster Bio Anti-KDM1/LSD1 KDM1A Rabbit Monoclonal Antibody catalog # M00532-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

KDM1A

Uniprot ID :

O60341

Immunogen :

A synthesized peptide derived from human KDM1 / LSD1

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Lysine-specific histone demethylase 1A

Synonyms :

Lysine-specific histone demethylase 1A; 1.-.-.-; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2; KDM1A; AOF2, KDM1, KIAA0601, LSD1;

Molecular Weight :

92903 MW

Protein Function :

Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E- cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.

Subcellular Localization :

Nucleus.

Tissue Specificity :

Ubiquitously expressed.

Research Category :

Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Methylation