Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10)

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10)

catalog :

M00456-2

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2H10

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M00456-2

Product Name :

Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

2H10

Host :

Mouse

Reactivity :

Human, Monkey

Application(s) :

Flow Cytometry, IF, IHC, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. Immunocytochemistry/Immunofluorescence, 2µg/ml. Flow Cytometry, 1-3μg/1x10^6 cells.

Description :

Boster Bio Anti-Beta 2 Microglobulin B2M Antibody Picoband™ (monoclonal, 2H10) catalog # M00456-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

B2M

Uniprot ID :

P61769

Immunogen :

E.coli-derived human Beta 2 Microglobulin recombinant protein (Position: Q22-M119). Human Beta 2 Microglobulin shares 69.4% and 74.5% amino acid (aa) sequence identity with mouse and rat Beta 2 Microglobulin, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG2b

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Gene Full Name :

beta-2-microglobulin

Synonyms :

Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3; B2M; CDABP0092, HDCMA22P

Protein Function :

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Subcellular Localization :

Secreted

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Background :

Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Research Category :

Cancer, Cardiovascular, Serum Proteins, Tumor Biomarkers