Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody | Boster M00434 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody

catalog :

M00434

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

FIG-20

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M00434

Product Name :

Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

FIG-20

Host :

Rabbit

Reactivity :

Human, Mouse

Application(s) :

Flow Cytometry, IF, IHC, ICC, WB

Application Details :

WB 1:1000-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .Flow Cytometry 1:50

Description :

Boster Bio Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody catalog # M00434. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

TOP1

Uniprot ID :

P11387

Immunogen :

A synthesized peptide derived from human TOP1

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

DNA topoisomerase 1

Synonyms :

DNA topoisomerase 1; 5.99.1.2; DNA topoisomerase I; TOP1;

Molecular Weight :

90726 MW

Protein Function :

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Subcellular Localization :

Nucleus, nucleolus. Nucleus, nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.

Tissue Specificity :

Endothelial cells

Research Category :

DNA / RNA, DNA Synthesis, Epigenetics and Nuclear Signaling