Anti-Fos Monoclonal Antibody (8B5) | Boster M00297-2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Fos Monoclonal Antibody (8B5)

catalog :

M00297-2

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8B5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry

product information

sku :

M00297-2

status :

Enabled

name :

Anti-Fos Monoclonal Antibody (8B5)

category name :

Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies

gene name :

FOS

price various sizes :

100ug / $418 100ug+Free HRP Secondary BA1050 / $418 100ug+Free Biotin Secondary BA1001 / $418

clonality :

Monoclonal

clone number :

8B5

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

description :

Mouse Monoclonal 8B5 antibody for FOS detection. Tested positive for ELISA, IHC, WB in Human, Mouse, Rat

short description :

Mouse Monoclonal 8B5 antibody for FOS detection. Tested positive for ELISA, IHC, WB in Human, Mouse, Rat

size :

100 ug

uniprot id :

P01100

host :

Mouse

immunogen :

Synthetic peptide corresponding to a portion of human c-Fos .

form :

Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

purification :

Thiophilic adsorption and size exclusion chromatography purified.

storage :

-20 C

application details :

ELISA (0.1ug/ml) . Immunohistochemistry (1:50) . Western Blot (0.5ug/ml, ECL) . Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

applications :

ELISA, IHC, WB

reactivity :

Human, Mouse, Rat

image labels :

Figure 2. IHC analysis of FOS using anti-FOS antibody (M00297-2). FOS was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FOS Antibody (M00297-2) overnight at 4 C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 1. Western blot analysis of FOS using anti-FOS antibody (M00297-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOS antigen affinity purified polyclonal antibody (Catalog # M00297-2) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for FOS.

research category :

Domain Families, Epigenetics And Nuclear Signaling, Hlh / Leucine Zipper, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Transcription, Transcription Factors

last modified :

2/6/19 22:57