Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody | Boster M00277 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody

catalog :

M00277

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

FOF-3

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

product information

SKU :

M00277

Product Name :

Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

FOF-3

Host :

Rabbit

Reactivity :

Human

Application(s) :

IP, IF, IHC, ICC, WB

Application Details :

WB 1:10000-1:20000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .IP 1:50

Description :

Boster Bio Anti-Chk2 CHEK2 Rabbit Monoclonal Antibody catalog # M00277. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

CHEK2

Uniprot ID :

O96017

Immunogen :

A synthesized peptide derived from human Chk2

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Serine/threonine-protein kinase Chk2

Synonyms :

Serine/threonine-protein kinase Chk2; 2.7.11.1; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2; CHEK2; CDS1, CHK2, RAD53;

Molecular Weight :

60915 MW

Protein Function :

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X- R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

Subcellular Localization :

Isoform 2: Nucleus. Isoform 10 is present throughout the cell.

Tissue Specificity :

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

Research Category :

Cancer, Cell Cycle, DNA / RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Kinases/Phosphatases