Anti-MDA5 Antibody (Monoclonal, 1B6) | Boster M00263 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

other

product name :

Anti-MDA5 Antibody (Monoclonal, 1B6)

catalog :

M00263

quantity :

100 ug/vial

product information

sku :

M00263

status :

Enabled

name :

Anti-MDA5 Antibody (Monoclonal, 1B6)

category name :

Primary Antibodies, Monoclonal Antibodies

gene name :

IFIH1

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1050 / $299 100ug+Free Biotin Secondary BA1001 / $299

clonality :

Monoclonal

clone number :

1B6

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Each vial contains 50% glycerol and 0.09% sodium azide.

description :

Monoclonal, 1B6 antibody for MDA5/IFIH1 detection. Host: Mouse.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. MDA5/IFIH1 information: Molecular Weight: 116689 MW; Subcellular Localization: Cytoplasm . Nucleus . May be found in the nucleus, during apoptosis; Tissue Specificity: Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.

short description :

Mouse Anti-MDA5 Antibody (Monoclonal, 1B6) for IFIH1 detectiontested for WB in Human, Mouse.

size :

100 ug/vial

uniprot id :

Q9BYX4

host :

Mouse

immunogen :

Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555).

form :

Liquid

purification :

Protein G-affinity purified.

storage :

At -20Â°C for one year. After reconstitution, at 4Â°C for one month. It can also be aliquotted and stored frozen at -20Â°C for a longer time. Avoid repeated freezing and thawing.

application details :

WB, 1:1, 000, ECL, Human, Mouse

applications :

reactivity :

Human, Mouse

image labels :

Western blot analysis of MDA5 expression in MW Marker (lane 1), HELA whole cell lysates (lane 2), A431 whole cell lysates (lane 3), L929 whole cell lysates (lane 4) and NIH3T3 whole cell lysates (lane 5), Jurkat whole cell lysates (lane 6). MDA5 at ~135KD was detected using mouse anti- MDA5 Antigen Affinity purified monoclonal antibody (Catalog # M00263) at 1: 1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001.

research category :

Antiviral Signaling, Cytokines, Dna / Rna, Epigenetics And Nuclear Signaling, Host Virus Interaction, Immune System Diseases, Immunology, Innate Immunity, Interferons, Interspecies Interaction, Microbiology, Rna Processing

synonyms :

Interferon-induced helicase C domain-containing protein 1;3.6.4.13;Clinically amyopathic dermatomyositis autoantigen 140 kDa;CADM-140 autoantigen;Helicase with 2 CARD domains;Helicard;Interferon-induced with helicase C domain protein 1;Melanoma differentiation-associated protein 5;MDA-5;Murabutide down-regulated protein;RIG-I-like receptor 2;RLR-2;RNA helicase-DEAD box protein 116;IFIH1;MDA5, RH116;

gene full name :

Interferon-induced helicase C domain-containing protein 1

molecular weight :

116689 MW

protein function :

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.

subcellular localization :

Cytoplasm . Nucleus . May be found in the nucleus, during apoptosis.

tissue specificity :

Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.

protein name :

Interferon-induced helicase C domain-containing protein 1 (IFIH1)

last modified :

2/16/19 1:26