Anti-Human Ki-67 Monoclonal Antibody | Boster M00254-2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Human Ki-67 Monoclonal Antibody

catalog :

M00254-2

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

monoclonal

reactivity :

human

application :

western blot, immunocytochemistry

product information

SKU :

M00254-2

Product Name :

Anti-Human Ki-67 Monoclonal Antibody

Size :

100 ul

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Application(s) :

IF, WB

Application Details :

WB: 1:1,000-5,000. IF 1:2,000-5,000

Description :

Boster Bio Anti-Human Ki-67 Monoclonal Antibody catalog # M00254-2. Tested in IF, WB applications. This antibody reacts with Human.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

MKI67

Uniprot ID :

P46013

Immunogen :

Recombinant construct containing the 2nd, 3rd and 4th Ki67 repeats of the human sequence (amino acids 1,111-1,490) expressed in and purified from E. coli.

Contents :

Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol, 5mM azide

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Proliferation marker protein Ki-67

Synonyms :

Proliferation marker protein Ki-67 ; Antigen identified by monoclonal antibody Ki-67 ; Antigen KI-67 ; Antigen Ki67 ; MKI67 ;

Molecular Weight :

358694 MW

Protein Function :

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Subcellular Localization :

Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).

Research Category :

Cancer, Cell Biology, Cell Cycle, Cell Division, Cell Type Marker, Cell Type Markers, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Tags & Cell Markers, Tumor Biomarkers