Anti-CD147/Emmprin Antibody Picoband™ (monoclonal, 7H5E7)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CD147/Emmprin Antibody Picoband™ (monoclonal, 7H5E7)

catalog :

M00248-5

quantity :

100 µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H5E7

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

M00248-5

Product Name :

Anti-CD147/Emmprin Antibody Picoband™ (monoclonal, 7H5E7)

Size :

100 µg/vial

Clonality :

Monoclonal

Clone Number :

7H5E7

Host :

Mouse

Reactivity :

Human, Mouse

Application(s) :

WB, IHC-P, IF, Flow Cytometry

Application Details :

Western blot, 0.25-0.5 µg/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse. Immunocytochemistry, 5 µg/ml, Human. Flow Cytometry, 1-3 μg/1x10^6 cells, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CD147/Emmprin Antibody Picoband™ (monoclonal, 7H5E7) catalog # M00248-5. Tested in Flow Cytometry, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Gene Name :

BSG

Uniprot ID :

P35613

Immunogen :

E.coli-derived human CD147/Emmprin recombinant protein (Position: E138-A323). Human CD147/Emmprin shares 51.1% and 51.9% amino acid (aa) sequence identity with mouse and rat CD147/Emmprin, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification :

Immunogen affinity purified.

Isotype :

IgG2a

Storage :

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Reconstitution :

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

Cluster of Differentiation 276 (CD276) or B7 Homolog 3 (B7-H3) is a human protein encoded by the CD276 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.