Anti-ATG5/Apg5 Rabbit Monoclonal Antibody | Boster M00240 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-ATG5/Apg5 Rabbit Monoclonal Antibody

catalog :

M00240

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

GCD-1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

product information

SKU :

M00240

Product Name :

Anti-ATG5/Apg5 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

GCD-1

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IP, IF, IHC, ICC, WB

Application Details :

WB 1:1000-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .IP 1:50

Description :

Boster Bio Anti-ATG5/Apg5 Rabbit Monoclonal Antibody catalog # M00240. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

ATG5

Uniprot ID :

Q9H1Y0

Immunogen :

A synthesized peptide derived from human ATG5

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Autophagy protein 5

Synonyms :

Autophagy protein 5; APG5-like; Apoptosis-specific protein; ATG5; APG5L, ASP;

Molecular Weight :

32447 MW

Protein Function :

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

Subcellular Localization :

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

Tissue Specificity :

Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Research Category :

Apoptosis, Associated Proteins, Cancer, Cardiovascular, Cell Biology, Cell Death, Intracellular, Metabolism, Metabolism Processes, Pathways And Processes, Proteasome / Ubiquitin, Proteolysis / Ubiquitin, Signal Transduction, Ub-Like Proteins