Anti-Vimentin Antibody | Boster M00235-5 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Vimentin Antibody

catalog :

M00235-5

quantity :

100 ul

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, bovine

application :

western blot, immunohistochemistry

product information

sku :

M00235-5

status :

Enabled

name :

Anti-Vimentin Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

VIM

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Supplied as an aliquot of IgY preparation plus 5mM NaN 3

description :

Anti-Vimentin Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse, cow, pig, horse, chicken

short description :

Anti-Vimentin Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse, cow, pig, horse, chicken

size :

100 ul

uniprot id :

P08670

host :

Chicken

immunogen :

Full length recombinant human vimentin protein , PROT-r-Vim , expressed in and purified from E. coli .

storage :

Stable at 4 C for at least one year

application details :

WB: 1:10000. IF/ICC and IHC: 1:5000.

applications :

IHC, WB

reactivity :

Bovine, Chicken, Equine, Human, Mouse, Pig, Rat

image labels :

Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, M00235-5, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. M00235-5 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells. Immunofluorescent analysis of HeLa cell culture stained with chicken pAb to vimentin, M00235-5, dilution 1:10,000 in green, and costained with Boster mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

research category :

Cancer, Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Developmental Biology, Intermediate Filaments, Intracellular, Lineage Markers, Lineage Specification, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Stem Cells, Tumor Biomarkers

synonyms :

Vimentin;VIM;

gene full name :

Vimentin

molecular weight :

53652 MW

protein function :

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

subcellular localization :

cytoplasm

tissue specificity :

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.

last modified :

2/6/19 22:57