Anti-Vimentin Antibody | Boster M00235-4 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Vimentin Antibody

catalog :

M00235-4

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry

product information

sku :

M00235-4

status :

Enabled

name :

Anti-Vimentin Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

VIM

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Antibody is supplied as an aliquot of serum

description :

Anti-Vimentin Antibody tested in WB, ICC, IF, IHC, reactive to Human, rat, mouse, cow, pig, horse

short description :

Anti-Vimentin Antibody tested in WB, ICC, IF, IHC, reactive to Human, rat, mouse, cow, pig, horse

size :

100 ul

uniprot id :

P08670

host :

Rabbit

immunogen :

Full length recombinant human vimentin protein , PROT-r-Vim , expressed in and purified from E. coli . expressed in and purified from E. coli.

storage :

Store at 4 for short term, for longer term at -20 . Avoid freeze / thaw cycles.

application details :

Western blot: 1:10,000. IF/ICC and IHC: 1:5,000.

applications :

IHC, WB

reactivity :

Bovine, Equine, Human, Mouse, Pig, Rat

image labels :

Western blot analysis of whole cell lysates using rabbit pAb to vimentin, M00235-4, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa. Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, M00235-4, dilution 1:5,000, in green, and mouse mAb to Actin, dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

research category :

Cancer, Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Developmental Biology, Intermediate Filaments, Intracellular, Lineage Markers, Lineage Specification, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Stem Cells, Tumor Biomarkers

synonyms :

Vimentin;VIM;

gene full name :

Vimentin

molecular weight :

53652 MW

protein function :

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

subcellular localization :

cytoplasm

tissue specificity :

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.

last modified :

2/6/19 22:57