Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3) | Boster M00232 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3)

catalog :

M00232

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SPVL3

reactivity :

human

application :

flow cytometry

product information

sku :

M00232

status :

Enabled

name :

Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3)

category name :

Primary Antibodies, Monoclonal Antibodies

gene name :

HLA-DQA1

price various sizes :

25ug sample size / $99 100 ug / $299 100 ug+Free HRP Secondary BA1050 / $299 100 ug+Free Biotin Secondary BA1001 / $299

clonality :

Monoclonal

clone number :

SPVL3

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide.

description :

Monoclonal clone# G2 antibody for HLA DQA1/HLA-DQA1 detection. Host: Mouse.Size: 100 ug. Tested applications: Flow Cytometry. Reactive species: Human. HLA DQA1/HLA-DQA1 information: Molecular Weight: 27805 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

short description :

Mouse IgG2a Kappa Monoclonal antibody for HLA-DQA1 detection. Tested with Flow Cytometry in Human. Various direct flourescent conjugates are available for FCM upon request. Please contact us for details.

size :

100 ug

sample size available :

25ug for $99, contact us for details

uniprot id :

P01909

host :

Mouse

immunogen :

Cells of T4 and CTL clone HG-38.

form :

Liquid

storage :

Store the antibody at 4 C, stable for 6 months. For long-term storage, store at -20 C. Avoid repeated freeze and thaw cycles.

isotype :

IgG2a Kappa

application details :

Flow Cytometry, 0.5-1 ug/10^6 cells.

applications :

Flow Cytometry

reactivity :

Human

image labels :

Cell Surface flow analysis of MHCII-DLADQ in PBMC (Lymphocyte gated) using 0.5 ug/10^6 cells. Green represents isotype control ; red represents anti-hMHCII-HLADQ antibody (Clone:SPVL3). Goat anti-mouse PE conjugated secondary antibody was used.

competitor equivalent skus :

sc 53312

research category :

Adaptive Immunity, Immunology

synonyms :

HLA class II histocompatibility antigen, DQ alpha 1 chain;DC-1 alpha chain;DC-alpha;HLA-DCA;MHC class II DQA1;HLA-DQA1;

gene full name :

HLA class II histocompatibility antigen, DQ alpha 1 chain

molecular weight :

27805 MW

protein function :

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

subcellular localization :

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single- pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

protein name :

HLA class II histocompatibility antigen, DQ alpha 1 chain

last modified :

2/6/19 22:57