Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) | Boster M00227-Biotin product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin)

catalog :

M00227-Biotin

quantity :

0.1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12D3D5

The same clone is also sold as:

OriGene: TA355043, TA355044

reactivity :

human

application :

western blot, ELISA

product information

SKU :

M00227-Biotin

Product Name :

Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin)

Size :

0.1 mg

Clonality :

Monoclonal

Clone Number :

12D3D5

Host :

Mouse

Reactivity :

Human, Mouse, Rat, Rabbit

Application(s) :

ELISA, WB

Application Details :

Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 µg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-GAPDH Monoclonal Antibody [12D3D5] (biotin) catalog # M00227-Biotin. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Concentration :

1 mg/mL

Gene Name :

GAPDH

Uniprot ID :

P04406

Immunogen :

Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.

Form :

Liquid

Contents :

Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Purification :

Biotin-GAPDH antibody is Protein A purified.

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

NADH dehydrogenase 2, mitochondrial

Synonyms :

NADH-ubiquinone oxidoreductase chain 2; NADH dehydrogenase subunit 2; Mtnd2; mt-Nd2; Nd2

Protein Function :

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Localization :

Mitochondrion inner membrane. Multi-pass membrane protein.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Background :

Mitochondrially encoded NADH dehydrogenase 2 is protein that in humans is encoded by the mitochondrial gene MT-ND2 gene. The ND2 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of MT-ND2 are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.