Anti-GAPDH Mouse Monoclonal Antibody | Boster M00227-7 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GAPDH Mouse Monoclonal Antibody

catalog :

M00227-7

quantity :

100 µl/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

17G14

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

product information

SKU :

M00227-7

Product Name :

Anti-GAPDH Mouse Monoclonal Antibody

Size :

100 µl/vial

Clonality :

Monoclonal

Clone Number :

17G14

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

WB, IHC, ICC, IF, IP, Flow Cytometry

Application Details :

WB 1:5000-1:20000.IHC 1:100-1:500.ICC/IF 1:100-1:250.IP 1:50.FC 1:50

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-GAPDH Mouse Monoclonal Antibody catalog # M00227-7. Tested in WB, IHC, ICC/IF, IP, FC applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

GAPDH

Uniprot ID :

P04406

Immunogen :

A synthesized peptide derived from human GAPDH Mouse Monoclonal

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Background :

p95 NBS1, also known as NBN or Nibrin, is a protein which in humans is encoded by the NBN gene. Nibrin is a protein associated with the repair of double strand breaks (DSBs) which pose serious damage to a genome. It is a 754 amino acid protein identified as a member of the NBS1/hMre11/RAD50(N/M/R, more commonly referred to asMRN) double strand DNA break repair complex. This complex recognizes DNA damage and rapidly relocates to DSB sites and forms nuclear foci. It also has a role in regulation of N/M/R (MRN) protein complex activity which includes end-processing of both physiological and mutagenic DNA double strand breaks (DSBs).