Anti-GAPDH Antibody (monoclonal, GAPDH-71.1) | Boster M00227-6 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GAPDH Antibody (monoclonal, GAPDH-71.1)

catalog :

M00227-6

quantity :

100µl/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GAPDH-71.1

The same clone is also sold as:

MilliporeSigma: G8795, G9295

reactivity :

human

application :

western blot, ELISA

product information

SKU :

M00227-6

Product Name :

Anti-GAPDH Antibody (monoclonal, GAPDH-71.1)

Size :

100µl/vial

Clonality :

Monoclonal

Clone Number :

GAPDH-71.1

Host :

Mouse

Reactivity :

Cattle, Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat

Application(s) :

ELISA, WB

Application Details :

WB: 1:100-500. ELISA: 1:500-1:5000

Description :

Boster Bio Anti-GAPDH Antibody (monoclonal, GAPDH-71.1) catalog # M00227-6. Tested in ELISA, WB applications. This antibody reacts with Cattle, Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat.

Gene Name :

GAPDH

Uniprot ID :

P04406

Immunogen :

Synthetic peptide CRLEKPAKYDDIKK coupled to KLH

Form :

Liquid

Isotype :

IgG1

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms :

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; 2.6.99.-; GAPDH; GAPD; CDABP0047, OK/SW-cl.12

Tissue Specificity :

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Background :

The enzyme glyceraldehydes-3-phosphate dehydrogenase is a tetramer of identical chains that catalyzes the reversible oxidative phosphorylation of glyceraldehydes-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide. This is an important energy-yielding step in carbohydrate metabolism. GAPDH is found in almost all species with low rate of evolutionary changes.