Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 | Boster M00227-5 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114

catalog :

M00227-5

quantity :

100 uL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

RM114

The same clone is also sold as:

Abnova: MAB12829, MAB14963
Novus Biologicals: NBP2-77402
OriGene: TA346868
Invitrogen: MA5-27911, MA5-27912

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

product information

SKU :

M00227-5

Product Name :

Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114

Size :

100 uL

Clonality :

Monoclonal

Clone Number :

RM114

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IP, ICC, WB

Application Details :

Western Blot (WB): 1:1000 dilution; Immunoprecipitation (IP): 1:200 dilution; Chromatin IP (ChIP): 1:200 dilution; Immunocytochemistry (ICC): 1:200 dilution; Immunohistochemistry (IHC): 1:200 dilution.

Description :

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 catalog # M00227-5. Tested in IP, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

GAPDH

Uniprot ID :

P04406

Immunogen :

A peptide corresponding to the C-terminus of GAPDH

Contents :

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms :

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; 2.6.99.-; GAPDH; GAPD; CDABP0047, OK/SW-cl.12;

Molecular Weight :

36053 MW

Protein Function :

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Subcellular Localization :

Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Research Category :

Alzheimer'S Disease, Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways And Processes, Signal Transduction