Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody

catalog :

M00227-3

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

monoclonal

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

M00227-3

Product Name :

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody

Size :

100 ul

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Equine

Application(s) :

IHC, WB, IF, ICC

Application Details :

WB: 1:1,000 IF/IHC: 1:100.

Description :

Boster Bio Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody catalog # M00227-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Equine.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

Gapdh

Uniprot ID :

P04797

Immunogen :

Full length protein purified from pig blood cells

Contents :

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN 3

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms :

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; 1.2.1.12; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH; 2.6.99.-; Gapdh; Gapd;

Molecular Weight :

35828 MW

Protein Function :

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity).

Subcellular Localization :

Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Nucleus. Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Colocalizes with CHP1 to small punctate structures along the microtubules tracks.

Tissue Specificity :

High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.

Research Category :

Alzheimer'S Disease, Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways And Processes, Signal Transduction