Anti-TNFAIP3/A20 Rabbit Monoclonal Antibody | Boster M00224 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-TNFAIP3/A20 Rabbit Monoclonal Antibody

catalog :

M00224

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

GCO-20

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M00224

Product Name :

Anti-TNFAIP3/A20 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

GCO-20

Host :

Rabbit

Reactivity :

Human

Application(s) :

Flow Cytometry, IF, IHC, ICC, WB

Application Details :

WB 1:1000-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .Flow Cytometry 1:50

Description :

Boster Bio Anti-TNFAIP3/A20 Rabbit Monoclonal Antibody catalog # M00224. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

TNFAIP3

Uniprot ID :

P21580

Immunogen :

A synthesized peptide derived from human TNFAIP3

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Tumor necrosis factor alpha-induced protein 3

Synonyms :

Tumor necrosis factor alpha-induced protein 3; TNF alpha-induced protein 3; 3.4.19.12; 6.3.2.-; OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20; A20p50; A20p37; TNFAIP3; OTUD7C;

Molecular Weight :

89614 MW

Protein Function :

Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS- induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.

Subcellular Localization :

Cytoplasm. Nucleus. Lysosome.

Research Category :

Cancer, Cytokines, Deubiquitination, Epigenetics and Nuclear Signaling, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Signal Transduction, Tnf Superfamily, Ubiquitin & Ubiquitin Like Modifiers