Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody | Boster M00189 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody

catalog :

M00189

quantity :

100 µl

price :

315 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AODE-1

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Boster product webpage

image

image 1 :

Western blot analysis of Argonaute 2 expression in (1) HeLa cell lysate; (2) RAW 2647 cell lysate; (3) C6 cell lysate (M00189). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO2 monoclonal antibody (Catalog # M00189) overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGO2

product information

SKU :

M00189

Product Name :

Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody

Price :

315 USD

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

AODE-1

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IP, IF, IHC, ICC, WB

Application Details :

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .IP 1:50 .Flow Cytometry 1:50

Description :

Boster Bio Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody catalog # M00189. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

AGO2

Uniprot ID :

Q9UKV8

Immunogen :

A synthesized peptide derived from human Argonaute 2

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Protein argonaute-2

Synonyms :

Protein argonaute-2 ; Argonaute2 ; hAgo2; 3.1.26.n2 ; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2 ; eIF-2C 2 ; eIF2C 2 ; PAZ Piwi domain protein; PPD; Protein slicer ; AGO2; EIF2C2;

Molecular Weight :

97208 MW

Protein Function :

Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Subcellular Localization :

Cytoplasm, P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.

Research Category :

ChIP’ing Antibodies, DNA / RNA, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, RNA Processing

more info or order :

Boster product webpage