antibody

Anti-DNMT1 Monoclonal Antibody

M00172-1

100 ug

monoclonal

mouse

nonconjugated

60B1220.1

human, mouse

western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation

Enabled

Anti-DNMT1 Monoclonal Antibody

Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies

DNMT1

100ug / $299 100ug+Free HRP Secondary BA1050 / $299 100ug+Free Biotin Secondary BA1001 / $299

Monoclonal

60B1220.1

No

Antibody concentration: 1 mg/ml, stored in PBS, 0.05% BSA, 0.05% sodium azide

Mouse Monoclonal antibody for DNMT1 detection. Tested positive for WB, IHC, IP, ChIP in Human; Mouse; Fish; Zebrafish.

Mouse Monoclonal antibody for DNMT1 detection. Tested positive for WB, IHC, IP, ChIP in Human; Mouse; Fish; Zebrafish.

100 ug

P26358

Mouse

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

liquid

Protein G Purified

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

WB, 1:1000 . IHC, 1:1000

ChIP, IP, IHC, WB

Human, Mouse

Figure 3. IHC analysis of DNMT1 using anti-DNMT1 antibody (M00172-1). DNMT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DNMT1 Antibody (M00172-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 1. IHC analysis of DNMT1 using anti-DNMT1 antibody (M00172-1). DNMT1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DNMT1 Antibody (M00172-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 2. Western blot analysis of DNMT1 using anti-DNMT1 antibody (M00172-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT1 antigen affinity purified polyclonal antibody (Catalog # M00172-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for DNMT1.

Chip'Ing Antibodies, Epigenetics And Nuclear Signaling

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