Anti-SLC2A1 Antibody Picoband™ (monoclonal, 10C10) | Boster M00163-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-SLC2A1 Antibody Picoband™ (monoclonal, 10C10)

catalog :

M00163-1

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10C10

The same clone is also sold as:

Invitrogen: MA5-49148

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

M00163-1

Product Name :

Anti-SLC2A1 Antibody Picoband™ (monoclonal, 10C10)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

10C10

Host :

Mouse

Reactivity :

Human

Application(s) :

Flow Cytometry, IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. Immunocytochemistry/Immunofluorescence, 5µg/ml. Flow Cytometry, 1-3μg/1x10^6 cells.

Description :

Boster Bio Anti-SLC2A1 Antibody Picoband™ (monoclonal, 10C10) catalog # M00163-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

SLC2A1

Uniprot ID :

P11166

Immunogen :

E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG1

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Gene Full Name :

solute carrier family 2 (facilitated glucose transporter), member 1

Synonyms :

Solute carrier family 2, facilitated glucose transporter member 1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; HepG2 glucose transporter; SLC2A1; GLUT1

Protein Function :

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

Subcellular Localization :

Cell membrane. Multi-pass membrane protein. Melanosome.

Tissue Specificity :

Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Background :

GLUT1, also known as SLC2A1, is a major glucose transporter in the mammalian blood-brain barrier whose gene is mapped to 1p35-p31.3 and contains 10 exons. It is present at high levels in primate erythrocytes and brain endothelial cells. Not only can transport dehydroascorbic acid (the oxidized form of vitamin C) into the brain, GLUT1 is also likely to contribute to HTLV-associated disorders through interacting with HTLV envelope glycoproteins. Functionally, GLUT1 deficiency causes a decrease in embryonic glucose uptake and apoptosis, which may be involved in diabetic embryopathy, by contrast, an increased expression of GLUT1 in some malignant tumors may suggest a role for glucose-derivative tracers to detect in vivo thyroid cancer metastases by positron-emission tomography scanning.

Research Category :

Channels, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Plasma Membrane, Signal Transduction