Anti-Nanog Rabbit Monoclonal Antibody | Boster M00153 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Nanog Rabbit Monoclonal Antibody

catalog :

M00153

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

EHD-14

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M00153

Product Name :

Anti-Nanog Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

EHD-14

Host :

Rabbit

Reactivity :

Human

Application(s) :

Flow Cytometry, IF, IHC, ICC, WB

Application Details :

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .Flow Cytometry 1:50

Description :

Boster Bio Anti-Nanog Rabbit Monoclonal Antibody catalog # M00153. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

NANOG

Uniprot ID :

Q9H9S0

Immunogen :

A synthesized peptide derived from human Nanog

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Homeobox protein NANOG

Synonyms :

Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog; NANOG;

Molecular Weight :

34620 MW

Protein Function :

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Subcellular Localization :

Nucleus.

Tissue Specificity :

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Research Category :

Cancer, Cell Cycle, Cell Differentiation, ChIP’ing Antibodies, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Intracellular, Nuclear Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Transcription Factors