Anti-CA2 Antibody Picoband™ (monoclonal, 10E11) | Boster M00143 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CA2 Antibody Picoband™ (monoclonal, 10E11)

catalog :

M00143

quantity :

100µg/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E+12

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

M00143

Product Name :

Anti-CA2 Antibody Picoband™ (monoclonal, 10E11)

Size :

100µg/vial

Clonality :

Monoclonal

Clone Number :

1E+12

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml. Immunocytochemistry/Immunofluorescence, 5µg/ml. Flow Cytometry, 1-3μg/1x10^6 cells.

Description :

Boster Bio Anti-CA2 Antibody Picoband™ (monoclonal, 10E11) catalog # M00143. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

CA2

Uniprot ID :

P00918

Immunogen :

E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Cross-reactivity :

No cross reactivity with other proteins.

Isotype :

IgG2b

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Gene Full Name :

Carbonic anhydrase 2

Synonyms :

Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II; CA2

Protein Function :

Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Subcellular Localization :

Cell membrane. Cytoplasm.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

Background :

CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.

Research Category :

Alzheimer'S Disease, Biochemicals, Cancer, Chemical Type, Diabetes, Heart Disease, Microbiology, Obesity