antibody

Anti-Bcl-6 Rabbit Monoclonal Antibody

M00142-1

100 µl

monoclonal

domestic rabbit

nonconjugated

BAF-2

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Anti-Bcl-6 Rabbit Monoclonal Antibody

100 µl

Monoclonal

BAF-2

Rabbit

Human

IP, IF, IHC, ICC, WB

WB 1:500-1:2000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .IP 1:50

Boster Bio Anti-Bcl-6 Rabbit Monoclonal Antibody catalog # M00142-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

BCL6

P41182

A synthesized peptide derived from human Bcl6

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Affinity-chromatography

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

B-cell lymphoma 6 protein

B-cell lymphoma 6 protein; BCL-6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; BCL6; BCL5, LAZ3, ZBTB27, ZNF51;

78846 MW

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6- binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4 (+) T-cells (T (FH) cells), promotes the expression of T (FH)-related genes but inhibits the differentiation of T (H)1, T (H)2 and T (H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53- dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH- dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Nucleus.

Expressed in germinal center T- and B-cells and in primary immature dendritic cells.

Cancer, Cancer Susceptibility, Domain Families, Epigenetics and Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Transcription, Transcription Factors, Zinc Finger