Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

other

product name :

Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody

catalog :

M00125

quantity :

100 ug/vial

product information

sku :

M00125

status :

Enabled

name :

Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Monoclonal Antibody

category name :

Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies

gene name :

PCNA

price various sizes :

20ug sample size / $99 100ug / $299 100ug+Free HRP Secondary BA1050 / $299 100ug+Free Biotin Secondary BA1001 / $299

clonality :

Monoclonal

clone number :

SPM350

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

description :

Mouse Monoclonal Antibody [Clone SPM350] antibody for PCNA detection. Host: Mouse.Size: 100ug/vial. Tested applications: IF. Reactive species: Chicken. PCNA information: Molecular Weight: 28769 MW; Subcellular Localization: Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

short description :

Anti-PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

size :

100 ug/vial

sample size available :

20ug for $99, contact us for details

uniprot id :

P12004

host :

Mouse

immunogen :

Rat PCNA/Protein A fusion protein

form :

Liquid

storage :

Antibody with azide - store at 2 to 8Â°C. Antibody without azide - store at -20 to -80Â°C. ,Antibody is stable for 24 months.

application details :

Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT). Immunofluorescence (0.5-1ug/ml)

applications :

IHC

reactivity :

Chicken, Drosophila, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish

image labels :

Formalin-fixed, paraffin-embedded human Tonsil stained withPCNA Monoclonal Antibody (SPM350)

competitor equivalent skus :

sc 65598 sc 56 sc 9857 sc 71858 sc 27912 sc 33756 sc 12849 sc 34005 sc 53408 sc 53407 sc 7907 sc 25280 sc 9857 R

research category :

Cancer, Cell Biology, Cell Cycle, Cell Division, Cell Type Markers, Chromatin Binding Proteins, Dna / Rna, Dna / Rna Binding, Dna Synthesis, Epigenetics And Nuclear Signaling, Tags & Cell Markers, Tumor Antigens, Tumor Biomarkers

synonyms :

Proliferating cell nuclear antigen;PCNA;Cyclin;PCNA;

gene full name :

Proliferating cell nuclear antigen

molecular weight :

28769 MW

protein function :

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

subcellular localization :

Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

last modified :

2/6/19 22:57