Anti-PCNA Rabbit Monoclonal Antibody | Boster M00125-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-PCNA Rabbit Monoclonal Antibody

catalog :

M00125-1

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

DO-16

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

product information

SKU :

M00125-1

Product Name :

Anti-PCNA Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

DO-16

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IP, IF, IHC, ICC, WB

Application Details :

WB 1:3000-1:10000 .IHC 1:50-1:200 .ICC/IF 1:100-1:500 .IP 1:50-1:100 .Flow Cytometry 1:200-1:500 .

Description :

Boster Bio Anti-PCNA Rabbit Monoclonal Antibody catalog # M00125-1. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

PCNA

Uniprot ID :

P12004

Immunogen :

A synthesized peptide derived from human PCNA

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Proliferating cell nuclear antigen

Synonyms :

Proliferating cell nuclear antigen; PCNA; Cyclin; PCNA;

Molecular Weight :

28769 MW

Protein Function :

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Subcellular Localization :

Nucleus. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Research Category :

Cell Biology, Cell Cycle, Cell Division, Cell Type Markers, Chromatin Binding Proteins, DNA / RNA, DNA / RNA Binding, DNA Synthesis, Epigenetics and Nuclear Signaling, Tags & Cell Markers, Tumor Antigens, Tumor Biomarkers