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Anti-PDGF Receptor Beta Antibody (Monoclonal, 42G12)

M00096

100 ug/vial

Enabled

Anti-PDGF Receptor Beta Antibody (Monoclonal, 42G12)

Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies

PDGFRB

100ug / $299 100ug+Free HRP Secondary BA1050 / $299 100ug+Free Biotin Secondary BA1001 / $299

Monoclonal

42G12

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

No

Each vial contains 0.09% sodium azide and 50% glycerol.

Monoclonal, 42G12 antibody for PDGF RECEPTOR BETA/PDGFRB detection. Host: Mouse.Size: 100ug/vial. Tested applications: IHC. Reactive species: Mouse. PDGF RECEPTOR BETA/PDGFRB information: Molecular Weight: 123968 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.

Mouse Anti-PDGF Receptor Beta Antibody (Monoclonal, 42G12) for PDGFRB detection tested with IHC (PS), WB in Mouse, Rat.

100 ug/vial

P09619

Mouse

Recombinant human PDGF Receptor beta .

Liquid

Protein G affinity purified.

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.

Weakly cross-reacts with human PDGF receptor beta.

WB, 1ug/ml, ECL, Mouse, Rat.

IHC, WB

Mouse, Rat

Western blot analysis of PDGF Receptor Beta expression in MW marker extract (lane 1) and mouse cardiac muscle extract (lane 2). PDGF Receptor Beta at 125KD was detected using mouse anti- PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 1 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001). PDGF Receptor Beta was detected in paraffin-embedded sections of human placenta tissues using mouse anti- PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 20 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021). PDGF Receptor Beta was detected in paraffin-embedded sections of human spleen tissues using mouse anti- PDGF Receptor Beta Antigen Affinity purified monoclonal antibody (Catalog # M00096) at 20 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1021).

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases, Vascular Inflammation

Platelet-derived growth factor receptor beta;PDGF-R-beta;PDGFR-beta;2.7.10.1;Beta platelet-derived growth factor receptor;Beta-type platelet-derived growth factor receptor;CD140 antigen-like family member B;Platelet-derived growth factor receptor 1;PDGFR-1;CD140b;PDGFRB;PDGFR, PDGFR1;

Platelet-derived growth factor receptor beta

123968 MW

Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.

Platelet-derived growth factor receptor beta (PDGFRB)

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