Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 | Boster M00074-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277

catalog :

M00074-1

quantity :

100 uL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

RM277

The same clone is also sold as:

Bio-Rad: MCA6319
Abnova: MAB14924
Invitrogen: MA5-44588

reactivity :

human

application :

western blot, immunohistochemistry

product information

SKU :

M00074-1

Product Name :

Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277

Size :

100 uL

Clonality :

Monoclonal

Clone Number :

RM277

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

WB, IHC

Application Details :

Immunohistochemistry (IHC): 1:1000-1:2000 dilution; Western Blot (WB): 1:500-1:1000 dilution.

Description :

Boster Bio Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 catalog # M00074-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

SMAD4

Uniprot ID :

Q13485

Immunogen :

A peptide corresponding to the C-terminus of human Smad4.

Contents :

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Mothers against decapentaplegic homolog 4

Synonyms :

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4, MADH4;

Molecular Weight :

60439 MW

Protein Function :

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed:25514493). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Subcellular Localization :

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD (PubMed:15799969). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).

Research Category :

Apoptosis, Cancer, Cancer Metabolism, Cardiogenesis, Cardiovascular, Cytoplasmic, Epigenetics and Nuclear Signaling, Hypertrophy, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Oncoproteins/Suppressors, Pathways And Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, Tgf Beta, Transcription Factors, Transcription Factors/Regulators, Tumor Suppressors, Vasculature