antibody

Anti-ER alpha ESR1 Rabbit Monoclonal Antibody

M00057-2

100 µl

monoclonal

domestic rabbit

nonconjugated

IE-5

human, mouse, rat, dogs

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation

Anti-ER alpha ESR1 Rabbit Monoclonal Antibody

100 µl

Monoclonal

IE-5

Rabbit

Dog, Human, Mouse, Rat

ChIP, Flow Cytometry, IF, IHC, ICC, WB

WB 1:500-1:2000 .IHC 1:50-1:100 .ICC/IF 1:50-1:100 .ChIP 1:30 .Flow Cytometry 1:30 .

Boster Bio Anti-ER alpha ESR1 Rabbit Monoclonal Antibody catalog # M00057-2. Tested in ChIP, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Dog, Human, Mouse, Rat.

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

ESR1

P03372

A synthesized peptide derived from human ER alpha

Liquid

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Affinity-chromatography

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Estrogen receptor

Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1; ESR, NR3A1;

66216 MW

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

Isoform 1: Nucleus. Cytoplasm. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. A minor fraction is associated with the inner membrane.

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

Cancer, ChIP’ing Antibodies, Endocrine System, Epigenetics and Nuclear Signaling, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway