Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody | Boster M00050-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody

catalog :

M00050-1

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

IDF-5

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M00050-1

Product Name :

Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

IDF-5

Host :

Rabbit

Reactivity :

Human

Application(s) :

Flow Cytometry, IF, IHC, ICC, WB

Application Details :

WB 1:500-1:1000 .IHC 1:50-1:200 .ICC/IF 1:50-1:200 .FCM 1:50

Description :

Boster Bio Anti-KMT6 / EZH2 Rabbit Monoclonal Antibody catalog # M00050-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

EZH2

Uniprot ID :

Q15910

Immunogen :

A synthesized peptide derived from human KMT6 / EZH2

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Histone-lysine N-methyltransferase EZH2

Synonyms :

Histone-lysine N-methyltransferase EZH2; 2.1.1.43; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6; EZH2; KMT6;

Molecular Weight :

85363 MW

Protein Function :

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

Subcellular Localization :

Nucleus.

Tissue Specificity :

Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.

Research Category :

Cancer, ChIP’ing Antibodies, Chromatin Modifying Enzymes, Chromatin Remodeling, Epigenetics and Nuclear Signaling, Methylation, Tumor Biomarkers