Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody

catalog :

M00045

quantity :

100 ug/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SPM225

The same clone is also sold as:

Abnova: MAB2544
Santa Cruz Biotechnology: sc-65617

reactivity :

human

application :

immunocytochemistry, immunohistochemistry - paraffin section

product information

SKU :

M00045

Product Name :

Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody

Size :

100 ug/vial

Clonality :

Monoclonal

Clone Number :

SPM225

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rabbit, Rat

Application(s) :

IF, IHC-P

Application Details :

Immunofluorescence (1-2ug/ml). Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

Description :

Boster Bio Anti-VEGF (Vascular Endothelial Growth Factor) VEGFA Monoclonal Antibody catalog # M00045. Tested in IF, IHC-P applications. This antibody reacts with Canine, Human, Mouse, Rabbit, Rat.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

VEGFA

Uniprot ID :

P15692

Immunogen :

Human VEGF189 recombinant protein

Form :

Liquid

Contents :

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Purification :

Protein A/G

Isotype :

IgG1, kappa

Storage :

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Gene Full Name :

Vascular endothelial growth factor A

Synonyms :

Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF;

Molecular Weight :

27042 MW

Protein Function :

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Subcellular Localization :

Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

Tissue Specificity :

Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Research Category :

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways And Processes, Response To Hypoxia, Signal Transduction