Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody

catalog :

M00023

quantity :

100 ug/vial

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GFR450

The same clone is also sold as:

OriGene: AM32821PU-S, AM32821PU-T
US Biological: 138540
Abnova: MAB11238
Novus Biologicals: NBP2-33068, NBP2-29440

reactivity :

human

application :

flow cytometry

product information

SKU :

M00023

Product Name :

Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody

Size :

100 ug/vial

Clonality :

Monoclonal

Clone Number :

GFR450

Host :

Mouse

Reactivity :

Human

Application(s) :

Flow Cytometry

Application Details :

User need to optimize the dilution ratio for this antibody

Description :

Boster Bio Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody catalog # M00023. Tested in Flow Cytometry applications. This antibody reacts with Human.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

EGFR

Uniprot ID :

P00533

Immunogen :

Recombinant extracellular domain of human EGFR protein

Form :

Liquid

Contents :

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.

Purification :

Protein A/G

Isotype :

IgG2a, kappa

Storage :

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Gene Full Name :

Epidermal growth factor receptor

Synonyms :

Epidermal growth factor receptor; 2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB, ERBB1, HER1;

Molecular Weight :

134277 MW

Protein Function :

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Subcellular Localization :

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).

Tissue Specificity :

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

Research Category :

Cancer, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tumor Biomarkers, Tyrosine Kinases