Anti-EGFR Rabbit Monoclonal Antibody | Boster M00023-4 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-EGFR Rabbit Monoclonal Antibody

catalog :

M00023-4

quantity :

100 µl/vial

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

1.7E+17

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

product information

SKU :

M00023-4

Product Name :

Anti-EGFR Rabbit Monoclonal Antibody

Size :

100 µl/vial

Clonality :

Monoclonal

Clone Number :

1.7E+17

Host :

Rabbit

Reactivity :

Human

Application(s) :

WB, ICC, IF, IP, Flow Cytometry

Application Details :

WB 1:1000-1:2000.ICC/IF 1:50-1:200.IP 1:30

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-EGFR Rabbit Monoclonal Antibody catalog # M00023-4. Tested in WB, ICC/IF, IP, FC applications. This antibody reacts with Human.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

EGFR

Uniprot ID :

P00533

Immunogen :

A synthesized peptide derived from human EGFR

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Background :

DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.