Anti-FoxP3 Purified Monoclonal Antibody | Boster M00011-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-FoxP3 Purified Monoclonal Antibody

catalog :

M00011-1

quantity :

0.1 mg (C100)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G3

The same clone is also sold as:

OriGene: TA336416, AM12131PU-N
Exbio: A4-601-C025, A4-601-C100, 1A-601-C025, 1P-601-C025, A6-601-C025, 11-601-C100, A6-601-C100, 11-601-C025, 1P-601-C100, 1A-601-C100
Santa Cruz Biotechnology: sc-52899
Abcam: ab187598, ab200568, ab210232
Novus Biologicals: NBP2-33297, NB100-56582
Tonbo Biosciences: 35-5773
Invitrogen: MA5-44011, MA5-18159, MA5-16222, MA1-10332, MA5-44084, MA1-41628, MA5-18160, MA5-16224
Abnova: MAB9852, MAB0107

reactivity :

human, mouse

application :

western blot, flow cytometry

product information

SKU :

M00011-1

Product Name :

Anti-FoxP3 Purified Monoclonal Antibody

Size :

0.1 mg (C100)

Clonality :

Monoclonal

Clone Number :

3G3

Host :

Mouse

Reactivity :

Human, Mouse

Application(s) :

Flow Cytometry, WB

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-FoxP3 Purified Monoclonal Antibody catalog # M00011-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse.

Concentration :

1 mg/ml

Gene Name :

FOXP3

Uniprot ID :

Q9BZS1

Immunogen :

Full-length His-tagged recombinant murine FoxP3

Form :

Liquid

Contents :

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Purification :

Purified by protein-A affinity chromatography

Isotype :

Mouse IgG1 kappa

Storage :

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

Gene Full Name :

tripartite motif containing 21

Synonyms :

E3 ubiquitin-protein ligase TRIM21; 52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING finger protein 81; RING-type E3 ubiquitin transferase TRIM21; Ro (SS-A); Sjoegren syndrome type A antigen; SS-A; Tripartite motif-containing protein 21; TRIM21; RNF81; RO52; SSA1

Protein Function :

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF (SKP2)-like complexes. A TRIM21-containing SCF (SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses.

Subcellular Localization :

Nucleus. Cytoplasm. Autophagosome. P-body.

Tissue Specificity :

Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.

Background :

Tripartite motif-containing protein 21 also known as E3 ubiquitin-protein ligase TRIM21 is a protein that in humans is encoded by the TRIM21 gene. It is mapped to 11p15.4. This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.