Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 | Boster M00006-3 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265

catalog :

M00006-3

quantity :

100 uL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

RM265

reactivity :

human, mouse

application :

western blot, immunohistochemistry

product information

sku :

M00006-3

status :

Enabled

name :

Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265

category name :

Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, IHC ICC IF Antibodies

gene name :

PTEN

clonality :

Monoclonal

clone number :

RM265

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

description :

Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 tested in WB, IHC, reactive to Human (Mouse)

short description :

Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 tested in WB, IHC, reactive to Human (Mouse)

size :

100 uL

uniprot id :

P60484

host :

Rabbit

immunogen :

A peptide corresponding to the C-terminus of human PTEN

storage :

-20 C

application details :

Immunohistochemistry (IHC): 1:1000-1:2000 dilution; Western Blot (WB): 1:1000-1:2000 dilution.

applications :

IHC, WB

reactivity :

Human, Mouse

image labels :

Figure 1. Western Blotting result. Western Blot of HeLa and 293 cells lysates using Anti-PTEN Rabbit Monoclonal Antibody (Clone RM265) at a 1:1000 dilution. Figure 2. IHC result. Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-PTEN Rabbit Monoclonal Antibody (Clone RM265) at a 1:1000 dilution.

research category :

Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Diabetes, Epigenetics And Nuclear Signaling, Kinases/Phosphatases, Lipid Signaling, Metabolism, Obesity, Oncoproteins/Suppressors, Signal Transduction, Signaling Pathway, Transcription, Tumor Suppressors

synonyms :

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;3.1.3.16 ;3.1.3.48 ;3.1.3.67 ;Mutated in multiple advanced cancers 1;Phosphatase and tensin homolog;PTEN;MMAC1, TEP1;

gene full name :

Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

molecular weight :

47166 MW

protein function :

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4 (PubMed:26504226). The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.

subcellular localization :

Cytoplasm . Nucleus . Nucleus, PML body . Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620). XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982).

tissue specificity :

Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

last modified :

2/8/19 6:55