Anti-TNF alpha Purified Monoclonal Antibody | Boster M00002-2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-TNF alpha Purified Monoclonal Antibody

catalog :

M00002-2

quantity :

0.1 mg (C100)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MAb11

The same clone is also sold as:

Exbio: 1F-688-T025, 1A-688-T025, 1P-688-T100, 1F-688-T100, 11-688-C025, 1A-688-T100, 1P-688-T025, 11-688-C100
BioLegend: 502943, 502961, 502925, 502906, 502901, 502928, 502931, 502959, 502960, 502932, 502912, 502908, 502945, 986802, 502917, 502923, 502916, 502903, 502909, 502927, 502935, 502929, 502902, 502930, 502915, 502956, 502946, 502938, 502954, 502924, 502953, 502947, 502904, 502950, 502949, 502913, 502957, 502939, 502920, 502926, 502940, 502944, 502948, 502962, 502955, 502936, 502937, 502941
Invitrogen: 416-7349-42, 606-7349-42, 404-7349-42, 11-7349-41, 48-7349-42, 13-7349-81, 14-7349-85, 25-7349-82, 367-7349-41, 12-7349-82, 56-7349-42, 45-7349-42, 48-7349-41, 11-7349-82, 53-7349-42, MA5-44111, MA5-44101, MA5-44021, 56-7349-41, 14-7349-81, 367-7349-42, 25-7349-41, MA5-44087, 13-7349-85, 606-7349-41, 11-7349-81, 17-7349-82, 61-7349-42, 404-7349-41, 416-7349-41, 12-7349-41, 17-7349-41
OriGene: AM26711RP-N, AM26711FC-N, AM26711PU-N, TA700026, AM26711AC-N
BD Biosciences: 559071, 554514, 558882, 560679, 561023, 552889, 560678, 557996, 554510, 554511, 561311, 554513, 557068, 562083, 560923, 562784, 562082, 557647, 554512, 559321

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

M00002-2

Product Name :

Anti-TNF alpha Purified Monoclonal Antibody

Size :

0.1 mg (C100)

Clonality :

Monoclonal

Clone Number :

MAb11

Host :

Mouse

Reactivity :

Pig, Human, Monkey

Application(s) :

Flow Cytometry, IHC, ICC, ELISA

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-TNF alpha Purified Monoclonal Antibody catalog # M00002-2. Tested in ELISA, Flow Cytometry, IHC, ICC applications. This antibody reacts with Human, Monkey, Pig.

Concentration :

1 mg/ml

Gene Name :

TNF

Uniprot ID :

P01375

Immunogen :

Recombinant human TNF-alpha

Form :

Liquid

Contents :

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Purification :

Purified by protein-A affinity chromatography

Isotype :

Mouse IgG1 kappa

Storage :

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

Gene Full Name :

vacuolar protein sorting 4 homolog B

Synonyms :

Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1

Protein Function :

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.

Subcellular Localization :

Late endosome membrane. Peripheral membrane protein. Prevacuolar compartment membrane.

Tissue Specificity :

Ubiquitously expressed.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

Vacuolar protein sorting-associated protein 4B is a protein that in humans is encoded by the VPS4B gene. It is mapped to 18q21.33. The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.