Anti-TNF alpha Purified Azide Free Monoclonal Antibody | Boster M00002-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-TNF alpha Purified Azide Free Monoclonal Antibody

catalog :

M00002-1

quantity :

0.1 mg (C100)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MAb1

The same clone is also sold as:

Invitrogen: MA5-46184, 14-7348-81, 16-7348-81, MA5-44020, 14-7348-85, 16-7348-85
BD Biosciences: 551220, 554508
OriGene: TA600026, AM20447PU-N, TA320488, AM20447AF-N
Exbio: 11-687-C100, 10-687-C100, 11-687-C025
BioLegend: 502801, 502805, 502806, 502802

reactivity :

human

application :

western blot, ELISA

product information

SKU :

M00002-1

Product Name :

Anti-TNF alpha Purified Azide Free Monoclonal Antibody

Size :

0.1 mg (C100)

Clonality :

Monoclonal

Clone Number :

MAb1

Host :

Mouse

Reactivity :

Human

Application(s) :

WB, ELISA

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-TNF alpha Purified Azide Free Monoclonal Antibody catalog # M00002-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Concentration :

1 mg/ml

Gene Name :

TNF

Uniprot ID :

P01375

Immunogen :

Recombinant human TNF-alpha

Form :

Liquid

Contents :

Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized

Purification :

Purified by protein-A affinity chromatography

Isotype :

Mouse IgG1 kappa

Storage :

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

Gene Full Name :

protein inhibitor of activated STAT 2

Synonyms :

E3 SUMO-protein ligase PIAS2; Androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; E3 SUMO-protein transferase PIAS2; Msx-interacting zinc finger protein; Miz1; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2; PIAS2; PIASX

Protein Function :

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.

Subcellular Localization :

Nucleus speckle. PML body. Nucleus.

Tissue Specificity :

Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Background :

E3 SUMO-protein ligase PIAS2 is an enzyme that in humans is encoded by the PIAS2 gene. It is mapped to 18q21.1. This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.