Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated | Boster H00227 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated

catalog :

H00227

quantity :

100 µl

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

A-7

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

H00227

Product Name :

Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated

Size :

100 µl

Clonality :

Monoclonal

Clone Number :

A-7

Host :

Rabbit

Reactivity :

Human, Monkey, Mouse, Rat

Application(s) :

Application Details :

WB 1:5000-1:20000

Description :

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated catalog # H00227. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Concentration :

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

GAPDH

Uniprot ID :

P04406

Immunogen :

A synthesized peptide derived from human GAPDH (HRP conjugated)

Form :

Liquid

Contents :

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Purification :

Affinity-chromatography

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms :

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; 2.6.99.-; GAPDH; GAPD; CDABP0047, OK/SW-cl.12;

Molecular Weight :

36053 MW

Protein Function :

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Subcellular Localization :

Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Research Category :

Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways And Processes, Signal Transduction