ELISA/assay

Human Granulysin PicoKine® Quick ELISA Kit

FEK1280

96 wells/kit, with removable strips.

499 USD

Human Granulysin PicoKine® Quick ELISA Kit

96 wells/kit, with removable strips.

499 USD

Human Granulysin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human GNLY in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Human

ELISA

GNLY

P22749

Expression system for standard: NS0; Immunogen sequence: M1-L145

There is no detectable cross-reactivity with other relevant proteins.

31.2 pg/ml - 2,000 pg/ml

<10 pg/ml

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

monoclonal antibody from mouse, polyclonal antibody from goat

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Granulysin

Granulysin;Lymphokine LAG-2;Protein NKG5;T-cell activation protein 519;GNLY;LAG2, NKG5, TLA519;

Granulysin

16374 MW

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Secreted. Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.

Expressed in natural killer and T-cells.

Granulysin is a substance released by cytotoxic T cells (CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.