Human PECAM-1/CD31 PicoKine® Quick ELISA Kit | Boster FEK0873 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human PECAM-1/CD31 PicoKine® Quick ELISA Kit

catalog :

FEK0873

quantity :

96 wells/kit, with removable strips.

product information

SKU :

FEK0873

Product Name :

Human PECAM-1/CD31 PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Description :

Human PECAM-1/CD31 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human PECAM-1/CD31 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

PECAM1

Uniprot ID :

P16284

Immunogen :

Expression system for standard: CHO; Immunogen sequence: Q28-K601

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Background :

Human platelet/endothelial cell adhesion molecule-1(PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 574 amino acids(Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.