Human CD163/M130 PicoKine® Quick ELISA Kit | Boster FEK0715 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CD163/M130 PicoKine® Quick ELISA Kit

catalog :

FEK0715

quantity :

96 wells/kit, with removable strips.

price :

499 USD

more info or order :

Boster product webpage

citations: 4

Reference
Wang J, Guo W, Du H, Yu H, Jiang W, Zhu T, et al. Elevated soluble CD163 plasma levels are associated with disease severity in patients with hemorrhagic fever with renal syndrome. PLoS ONE. 2014;9:e112127 pubmed publisher
Kallestrup M, MÃ¸ller H, Tankisi H, Andersen H. Soluble CD163 levels are elevated in cerebrospinal fluid and serum in people with Type 2 diabetes mellitus and are associated with impaired peripheral nerve function. Diabet Med. 2015;32:54-61 pubmed publisher
Rojo Martinez G, Maymó Masip E, Rodriguez M, Solano E, Goday A, Soriguer F, et al. Serum sCD163 levels are associated with type 2 diabetes mellitus and are influenced by coffee and wine consumption: results of the Di@bet.es study. PLoS ONE. 2014;9:e101250 pubmed publisher
Jasiewicz M, Kowal K, Kowal Bielecka O, Knapp M, Skiepko R, Bodzenta Lukaszyk A, et al. Serum levels of CD163 and TWEAK in patients with pulmonary arterial hypertension. Cytokine. 2014;66:40-5 pubmed publisher

image

image 1 :

Human CD163 PicoKine ELISA Kit standard curve

product information

SKU :

FEK0715

Product Name :

Human CD163/M130 PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Price :

499 USD

Description :

Human CD163 / M130 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CD163

Uniprot ID :

Q86VB7

Immunogen :

Expression system for standard: NS0; Immunogen sequence: G41-S1045

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

1.56 ng/ml - 100 ng/ml

Sensitivity :

<150 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Scavenger receptor cysteine-rich type 1 protein M130

Synonyms :

Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130;

Protein Name :

Scavenger receptor cysteine-rich type 1 protein M130

Molecular Weight :

125451 MW

Protein Function :

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

Subcellular Localization :

Soluble CD163: Secreted.

Tissue Specificity :

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

Sequence Similarities :

Contains 9 SRCR domains.

Background :

CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

more info or order :

Boster product webpage