ELISA/assay

Human B7-1/CD80 PicoKine® Quick ELISA Kit

FEK0707

96 wells/kit, with removable strips.

499 USD

Human B7-1/CD80 PicoKine® Quick ELISA Kit

96 wells/kit, with removable strips.

499 USD

Human B7-1/CD80 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD80 in cell culture supernatants and serum. Sensitivity: 10pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Human

ELISA

CD80

P33681

Expression system for standard: NS0; Immunogen sequence: V35-N242

There is no detectable cross-reactivity with other relevant proteins.

62.5 pg/ml - 4,000 pg/ml

<10 pg/ml

cell culture supernatants and serum

monoclonal antibody from mouse, monoclonal antibody from mouse

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

T-lymphocyte activation antigen CD80

T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7;

T-lymphocyte activation antigen CD80

33048 MW

Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

Membrane; Single-pass type I membrane protein.

Expressed on activated B-cells, macrophages and dendritic cells.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.