Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit | Boster FEK0644 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit

catalog :

FEK0644

quantity :

96 wells/kit, with removable strips.

price :

499 USD

more info or order :

Boster product webpage

citations: 1

Reference
Kopczynska E, Dancewicz M, Kowalewski J, Makarewicz R, Kardymowicz H, Kaczmarczyk A, et al. Influence of surgical resection on plasma endoglin (CD105) level in non‑small cell lung cancer patients. Exp Oncol. 2012;34:53-6 pubmed

image

image 1 :

Human CD105 PicoKine ELISA Kit standard curve

product information

SKU :

FEK0644

Product Name :

Human Endoglin/CD105/ENG PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Price :

499 USD

Description :

Human CD105 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ENG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Human

Application :

ELISA

Gene Name :

ENG

Uniprot ID :

P17813

Immunogen :

Expression system for standard: CHO; Immunogen sequence: E26-G586

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<15 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Endoglin

Synonyms :

Endoglin;CD105;ENG;END;

Protein Name :

Endoglin

Molecular Weight :

70578 MW

Protein Function :

Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Endoglin is restricted to endothelial cells in all tissues except bone marrow.

Background :

CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

more info or order :

Boster product webpage