Mouse TNF Alpha/Tumor necrosis factor PicoKine® Quick ELISA Kit

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse TNF Alpha/Tumor necrosis factor PicoKine® Quick ELISA Kit

catalog :

FEK0527

quantity :

96 wells/kit, with removable strips.

product information

SKU :

FEK0527

Product Name :

Mouse TNF Alpha/Tumor necrosis factor PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Description :

Mouse TNF Alpha/Tumor necrosis factor PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

CST3

Uniprot ID :

P06804

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: L80-L235

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml (cell culture supernatants), 7.8 pg/ml - 500 pg/ml (mouse serum, plasma)

Sensitivity :

<1 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from hamster, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

tumor necrosis factor

Synonyms :

TNF-alpha APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Protein Name :

Tumor necrosis factor

Background :

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte- macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Research Category :

Atherosclerosis, Cardiovascular, Caspases Etc, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated