Human TIMP-1 PicoKine® Quick ELISA Kit | Boster FEK0520 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TIMP-1 PicoKine® Quick ELISA Kit

catalog :

FEK0520

quantity :

96 wells/kit, with removable strips.

price :

499 USD

more info or order :

Boster product webpage

citations: 1

Reference
Dhir V, Srivastava R, Aggarwal A. Circulating Levels of Soluble Receptor Activator of NF- κ B Ligand and Matrix Metalloproteinase 3 (and Their Antagonists) in Asian Indian Patients with Ankylosing Spondylitis. Int J Rheumatol. 2013;2013:814350 pubmed publisher

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product information

SKU :

FEK0520

Product Name :

Human TIMP-1 PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Price :

499 USD

Description :

Human TIMP-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TIMP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 5pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Human

Application :

ELISA

Gene Name :

TIMP1

Uniprot ID :

P01033

Immunogen :

Expression system for standard: NS0; Immunogen sequence: C24-A207

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<5 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Metalloproteinase inhibitor 1

Synonyms :

Metalloproteinase inhibitor 1;Erythroid-potentiating activity;EPA;Fibroblast collagenase inhibitor;Collagenase inhibitor;Tissue inhibitor of metalloproteinases 1;TIMP-1;TIMP1;CLGI, TIMP;

Protein Name :

Metalloproteinase inhibitor 1

Molecular Weight :

23171 MW

Protein Function :

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

Subcellular Localization :

Secreted .

Tissue Specificity :

Detected in rheumatoid synovial fluid (at protein level).

Sequence Similarities :

Belongs to the protease inhibitor I35 (TIMP) family.

Background :

The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.

more info or order :

Boster product webpage