Mouse P-Selectin / CD62P PicoKine® Quick ELISA Kit | Boster FEK0506 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse P-Selectin / CD62P PicoKine® Quick ELISA Kit

catalog :

FEK0506

quantity :

96 wells/kit, with removable strips.

product information

SKU :

FEK0506

Product Name :

Mouse P-Selectin / CD62P PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Description :

Mouse P-Selectin / CD62P PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Selp in cell culture supernatants, serum and plasma (citrate). Sensitivity: 5pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Mouse

Application :

ELISA

Gene Name :

Selp

Uniprot ID :

Q01102

Immunogen :

Expression system for standard: NS0; Immunogen sequence: W42-A709

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<5 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (citrate)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

P-selectin

Synonyms :

P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-external membrane protein;PADGEM;CD62P;Selp;Grmp;

Protein Name :

P-selectin

Molecular Weight :

83099 MW

Protein Function :

Ca (2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Sequence Similarities :

Belongs to the selectin/LECAM family.

Background :

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.