Human sL-Selectin PicoKine® Quick ELISA Kit | Boster FEK0503 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human sL-Selectin PicoKine® Quick ELISA Kit

catalog :

FEK0503

quantity :

96 wells/kit, with removable strips.

price :

499 USD

more info or order :

Boster product webpage

citations: 1

Reference
MacKinnon J, Knott R, Forrester J. Altered L-selectin expression in lymphocytes and increased adhesion to endothelium in patients with diabetic retinopathy. Br J Ophthalmol. 2004;88:1137-41 pubmed

image

image 1 :

Human sL-Selectin PicoKine ELISA Kit standard curve

product information

SKU :

FEK0503

Product Name :

Human sL-Selectin PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Price :

499 USD

Description :

Human sL-Selectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SELL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Human

Application :

ELISA

Gene Name :

SELL

Uniprot ID :

P14151

Immunogen :

Expression system for standard: NS0; Immunogen sequence: W39-N332

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<5 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

L-selectin

Synonyms :

L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte surface antigen Leu-8;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;TQ1;gp90-MEL;CD62L;SELL;LNHR, LYAM1;

Protein Name :

L-selectin

Molecular Weight :

42187 MW

Protein Function :

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Expressed in B-cell lines and T-lymphocytes.

Sequence Similarities :

Belongs to the selectin/LECAM family.

Background :

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin)is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

more info or order :

Boster product webpage