Mouse sE-Selectin PicoKine® Quick ELISA Kit | Boster FEK0502 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse sE-Selectin PicoKine® Quick ELISA Kit

catalog :

FEK0502

quantity :

96 wells/kit, with removable strips.

product information

SKU :

FEK0502

Product Name :

Mouse sE-Selectin PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Description :

Mouse sE-Selectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Sele in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Mouse

Application :

ELISA

Gene Name :

Sele

Uniprot ID :

Q00690

Immunogen :

Expression system for standard: NS0; Immunogen sequence: W22-P557

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, citrate)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

E-selectin

Synonyms :

E-selectin;CD62 antigen-like family member E;Endothelial leukocyte adhesion molecule 1;ELAM-1;Leukocyte-endothelial cell adhesion molecule 2;LECAM2;CD62E;Sele;Elam-1;

Protein Name :

E-selectin

Molecular Weight :

66750 MW

Protein Function :

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

Subcellular Localization :

Cell membrane ; Single-pass type I membrane protein.

Sequence Similarities :

Belongs to the selectin/LECAM family.

Background :

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.