Mouse GM-CSF PicoKine® Quick ELISA Kit | Boster FEK0365 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse GM-CSF PicoKine® Quick ELISA Kit

catalog :

FEK0365

quantity :

96 wells/kit, with removable strips.

price :

499 USD

more info or order :

Boster product webpage

citations: 1

Reference
He S, Sun K, Wang L, Li D, Zhang Y. [GM-CSF gene-modified dendritic cell vaccine enhances antitumor immunity in vitro]. Zhonghua Zhong Liu Za Zhi. 2010;32:410-4 pubmed

image

image 1 :

Mouse GM-CSF PicoKine ELISA Kit standard curve

product information

SKU :

FEK0365

Product Name :

Mouse GM-CSF PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Price :

499 USD

Description :

Mouse GM-CSF PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Csf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Mouse

Application :

ELISA

Gene Name :

Csf2

Uniprot ID :

P01587

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: A18-K141

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<1 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Granulocyte-macrophage colony-stimulating factor

Synonyms :

Granulocyte-macrophage colony-stimulating factor;GM-CSF;Colony-stimulating factor;CSF;Csf2;Csfgm;

Protein Name :

Granulocyte-macrophage colony-stimulating factor

Molecular Weight :

16091 MW

Protein Function :

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subcellular Localization :

Secreted.

Sequence Similarities :

Belongs to the GM-CSF family.

Background :

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa.

more info or order :

Boster product webpage