Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit

catalog :

FEK0362

quantity :

96 wells/kit, with removable strips.

price :

499 USD

more info or order :

Boster product webpage

citations: 1

Reference
Wiesenhofer B, Stockhammer G, Kostron H, Maier H, Hinterhuber H, Humpel C. Glial cell line-derived neurotrophic factor (GDNF) and its receptor (GFR-alpha 1) are strongly expressed in human gliomas. Acta Neuropathol. 2000;99:131-7 pubmed

image

image 1 :

Human GDNF PicoKine ELISA Kit standard curve

product information

SKU :

FEK0362

Product Name :

Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Price :

499 USD

Description :

Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Human

Application :

ELISA

Gene Name :

GDNF

Uniprot ID :

P39905

Immunogen :

Expression system for standard: NS0; Immunogen sequence: R109-I211

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<4 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Glial cell line-derived neurotrophic factor

Synonyms :

Glial cell line-derived neurotrophic factor;hGDNF;Astrocyte-derived trophic factor;ATF;GDNF;

Protein Name :

Glial cell line-derived neurotrophic factor

Molecular Weight :

23720 MW

Protein Function :

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular Localization :

Secreted .

Tissue Specificity :

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.

Sequence Similarities :

Belongs to the TGF-beta family. GDNF subfamily.

Background :

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.

more info or order :

Boster product webpage